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Determination of tocopherols and tocotrienols content in vegetable oils and industrial fats




Key words:

tocochromanols, vegetable oils, bioactive compounds, HPLC.

Vegetable oils and fats contains several bioactive substances possessing beneficial effects on human health. This is the case of the high level of unsaturated fatty acids and a pool of minor compounds with a powerful antioxidant activity such as tocopherols and tocotrienols, collectively called tocochromanols, belonging to group of vitamin E. Vegetable oils are good sources of vitamin E for the human. The average concentration of tocochromanols is ten-fold higher than in other plants. The level of those compounds in oils mainly depends on the quality, the type, and the variety of raw substances.
The aim of this study was the determination tocopherols and tocotrienols content in selected oils and fats with the usage of HPLC equipped with a fluorescence detector.
The analysis was based on products bought in supermarkets in Poznan and those obtained directly from the manufacturers. Analysis of tocochromanols in seven cold-pressed oils and three industrial fats was done. Investigation showed that α- and ɣ- tocopherol were the major homologues in vegetable oils and fats. The highest content of total tocopherols was found in sunflower oil (788.1 μg/g) and the main vitamer was α-tocopherol. The lowest tocopherols level and simultaneously the highest tocotrienols content was found in black caraway oil.
The main tocotrienol found in it was β-tocotrienol (156.9 μg/g). In analysed margarines the major homologues were α- and ɣ- tocotrienol. In evening primrose, milk thistle, rapeseed, linseed and chia seeds oils were not detected none of the four tocotrienol vitamers.

dr Krzysztof PRZYGOŃSKI
Department of Food Concentrates and Starch Product
Prof. Wacław Dąbrowski Institute of Agricultural
and Food Biotechnology
40 Starołęcka Str., 61-361, Poznań, Poland
phone: (+48) 61-8731-989
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.




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