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Direct measurement of antiradical capacity of oilseed cakes by DPPH assay using the quencher approach

Key words:
antioxidants, antiradical capacity,
oilseeds, QUENCHER, by-products.

The oilseed by-products are the sources of substances with antioxidant potential, including
antiradical capacity. As the extract is not representative of the antioxidant capacity of plant
material, the novel approach called QUENCHER was developed by Serpen et al. It is based on
the direct measurement of absorbance after mixing the solid samples with the free radical,
which doesn’t require the preparation of the extract.
The aim of this study was to evaluate the antiradical activity of oilseed cakes using the
direct QUENCHER procedure. The material was obtained from: coconut, peanut, walnut,
sesame, black cumin, evening primrose, flaxseed, pumpkin seeds, and sunflower seeds. The
ground cakes were diluted with powdered cellulose and mixed with ethanolic solution of
DPPH (2,2-diphenyl-1-picrylhydrazyl). The absorbance of supernatants was measured at
λ=515 nm. In parallel, the oilseed cakes were extracted with 80% ethanol and the antiradical
activity of the extracts against DPPH was measured.
The results of the study proved the antiradical activity of oilseed cakes. The highest
antiradical properties assessed by the QUENCHER procedure were found in the evening
primrose and walnut cakes (134.6 and 128.5 μmol Trolox/g d.m., respectively), followed by
sunflower seeds, black cumin and peanut residues. The results obtained with the use of the
direct procedure gave higher values, showing that the antiradical potential of plant material is
greater than that measured in the extract.

dr inż. Urszula SAMOTYJA
prof. dr hab. Maria MAŁECKA
dr inż. Maria SIELICKA
Poznań University of Economics and Business
Faculty of Commodity Science
Department of Food Commodity Science
al. Niepodległości 10, 61-875 Poznań, Poland
e-mail: This email address is being protected from spambots. You need JavaScript enabled to view it.

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